by Biostatsquid | Aug 24, 2025 | Learning, Statistics
How to interpret ANOVA (analysis of variance) easily explained! When you’re working with data and want to determine whether different groups have significantly different averages, simply eyeballing the numbers won’t cut it. That’s where ANOVA (Analysis of...
by Biostatsquid | Jul 15, 2025 | RNAseq, scRNAseq, Tutorials, Uncategorized
Hey! You’re looking at an old post. Newer version here: clusterProfiler tutorial in R R TUTORIAL: Perform pathway enrichment analysis with clusterProfiler() in R Table of contents 5 Before you start 5 Step-by-step clusterProfiler tutorial 9 Step 0: Set up your...
by Biostatsquid | Jul 15, 2025 | scRNAseq, Tutorials
Hey! You’re looking at an old post. Newer version here: fgsea tutorial in R A step-by-step R tutorial on performing Gene Set Enrichment Analysis with R package fgsea In this tutorial you will learn how to conduct Gene Set Enrichment Analysis (GSEA) using...
by Biostatsquid | Jun 24, 2025 | Statistics
Setting thresholds for differential gene expression (DGE) analysis is crucial and depends on several factors. In essence, for a list of genes, we are trying to define what counts as biologically meaningful versus just statistically significant. The question is…...
by Biostatsquid | Jun 6, 2025 | Learning, Statistics, Statistics in R, Tutorials
When working with biological data, we often want to compare measurements across multiple groups. However, these measurements aren’t always normally distributed. In such cases, non-parametric methods like the Kruskal-Wallis test and Dunn’s post-hoc test are ideal...